Fig. 5
Comparison of drug-metabolizing activities of various CYPs between ELC-org-mono and primary cryopreserved human small intestinal cells. Drug-metabolizing activities of CYP3A4, CYP2D6, CYP2C9, and CYP2C19 between ELC-org-mono and primary cryopreserved human small intestinal cells were examined by quantifying the metabolites of each substrate (MDOH, TSOH, BFOH, DFOH and MPOH) by UPLC-MS/MS analysis. In the case of CYP3A4, midazolam and testosterone were used as substrates. Drug-metabolizing activities in primary cryopreserved human small intestinal cells were measured just after the cells were thawed. All data represent the mean ± S.D. (n = 3, biological replicates)