Fig. 1

Systemic administration of 3R4F suppresses HSC niche function of mBM-MSCs. (A) Schematic outline of systemic 3R4F administration and subsequent CD34⁺ HSPC transplantation in humanized mice. (B) Engraftment of human cells was determined from the peripheral blood of 3R4F-treated humanized mice by flow cytometric analysis. Left: representative dot plot images. Right: histogram for repetitive results (n = 4). (C) Schematic outline of the isolation of 3R4F-exposed mBM-MSCs and the in vitro BM niche function test. CD34⁺ cells were isolated from hUCB and cocultured with 3R4F-treated mBM-MSCs. (D) Body weight change was measured for 12 days. Body weights were normalized using the weight on Day 1 as the denominator (n = 10 mice/group). (E) After 3 days of coculture with 3R4F-exposed mBM-MSCs, the CD34⁺CD90⁺ HSPC population in CD45⁺ cells was analyzed using flow cytometry. Left: representative dot plot images. Right: histogram for repetitive results (n = 3). Experiments were performed at least three times. Data are presented as the mean ± S.D. (**p < 0.01)