Fig. 1
From: Monocytes prevent apoptosis of iPSCs and promote differentiation of kidney organoids
CHIR induces apoptosis of iPSC. A iPSC live cell number after 48 h of stimulation with different concentrations of CHIR or noggin was assessed using CCK8 kit. B Cellular level of ROS in the presence of different concentrations of CHIR and noggin separately and together was analyzed 24 h after their addition using CellRox green reagent. Concentrations of CHIR is in µM, concentrations of Noggin is in ng/mL. C Mitochondrial superoxide production in the presence of different concentrations of CHIR and noggin separately and together was analyzed 24 h after their addition using MitoSOX green reagent. Concentrations of CHIR is in µM, concentrations of Noggin is in ng/mL. D iPSC treated with different concentrations of CHIR in the presence of 5 ng/ml Noggin were lysed and expression of cleaved caspase 3, cleaved PARP-1 was assessed by western blotting. Full-length blots are shown in Additional file 1 Fig. S2