Fig. 2

Construction of the in vitro hematopoietic organoids. a Schematic diagram of the construction of the hematopoietic organoids. b Immunofluorescence images of cells in GelMA under different conditions (scale bar, 50 µm). c Various niche cell-specific protein immunofluorescence staining in GelMA with niche cell groups (scale bar, 20 µm). d Flow cytometry analysis of CD34 and CD45 expression in cells for each condition. e Percentage and number of CD34⁺CD45⁺ cells on day 7 for each condition. Results are presented as the mean ± SD from three independent experiments. Unpaired Student’s t-test, *p < 0.05. f Giemsa staining of megakaryocytes, promyelocytes, and erythroblasts from GelMA with niche cells group on day 7 (green: megakaryocytes; blue: promyelocytes; red: erythroblasts. scale bar, 10 µm). g Immunofluorescence staining showed the expression of specific proteins associated with megakaryocytes (CD41 and PF4), erythroid cells (CD235a and γ-globin), and granulocytes (NP57 and MPO) when co-cultured with niche cells. Scale bars, 10 μm. h Percentage and number of CD41⁺CD42b⁺, CD45⁺CD11b⁺ and CD71⁺CD235a⁺ cells on day 7 for each condition. Results are presented as the mean ± SD from three independent experiments. Unpaired Student’s t-test, *p < 0.05; **p < 0.01; ***p < 0.001