Fig. 3

Construction of the in vitro radiation injury model based on the hematopoietic organoids. a Schematic diagram demonstrates the organoids from -Niche cell and + Niche cell groups received radiation at 0 or 4 Gy. b Comparative analysis of CXCL12 concentration in the two groups under different conditions one day after radiation exposure. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, **p < 0.01; ***p < 0.001;###p < 0.001. c Flow cytometry analysis of the percentage of caspase-3-positive cells in CD45⁺ cell population from the two groups one day after radiation exposure. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, ***p < 0.001. d Flow cytometry analysis of the percentage of caspase-3-positive cells in CD34⁺CD45⁺ cell population from the two groups one day after radiation exposure. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, ***p < 0.001. e Percentage of CD34⁺CD45⁺ cells on the first and fourth day after 4 Gy irradiation. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, ***p < 0.001. f Schematic diagram of the construction of the radiation injury model. g ROS levels in CD45⁺ and CD34⁺CD45⁺ cells under different radiation doses. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001. h γ-H2AX expression levels in CD45⁺ cells under different radiation doses. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, *p < 0.05. I. γ-H2AX expression levels in CD34⁺CD45⁺ cells under different radiation doses. Results are presented as the mean ± SD from three independent experiments. One-way ANOVA, *p < 0.05