Fig. 6

Combined ADSCs and SDSCs inhibited chondrocyte degradation and enhanced autophagy. A chondrocyte micromass by Alcian Blue staining. Cell immunofluorescence (B) and Western blot (C) for Col2 and MMP13 were quantitatively analyzed by gray value (D) (n = 3). E the levels of inflammatory factors IL-10 and TNF-α and IL-1β were determined by ELISA. F TEM analysis showing autophagosomes (arrowed). G Cell immunofluorescence for LC3II  and J the proportion of cells with LC3B puncta. Western blot (H) for LC3II/I and P62 were quantitatively analyzed by gray value (K). I, L autophagosome in chondrocytes by MDC staining and puncta were quantitated (n = 25). All data are shown as the mean ± SD. ^#denotes compared with control, ^##P < 0.01，^###P < 0.001; * denotes compared with IL-1β, *P < 0.05, **P < 0.01, ***P < 0.001