Fig. 2

Effect of AA treatment in MSC priming during expansion on MSC metabolic profile. (A) MSC glycolytic function represented by real-time extracellular acidification rate (ECAR) plot of Untreated and AA-treated MSCs from Donor 1 in response to glucose, oligomycin and 2-deoxyglucose (2-DG) in Seahorse XF Glycolysis Stress test. (B) Energy map of Untreated and AA-treated MSCs presenting both ECAR and oxygen consumption rate (OCR) at the basal level. (C) OCR: ECAR ratio of Untreated and AA-treated MSCs at the basal level. (D) Glucose consumption and (E) lactate production rates were measured from the changes in the glucose and lactate concentrations in fresh and spent media, normalized to the total number of cells and hours of incubation. Experiments were performed in 3 technical replicates. Data are presented as mean ± standard deviation. * P < 0.05 and ** P < 0.01 compared to Untreated