Fig. 1
Characteristics of Ad-MSCs, BM-MSCs, and XF-iMSCs. a Bright-field morphological images of Ad-MSCs, BM-MSCs, and XF-iMSCs. b Representative immunofluorescence images of Ad-MSCs, BM-MSCs, and XF-iMSCs stained with anti-COL6 antibodies. c mRNA expression of COL6A1. The mRNA expression level of each gene in Ad-MSCs, BM-MSCs, and XF-iMSCs was analyzed using RT-qPCR. Levels are shown relative to those in XF-iMSCs. Data are shown as the mean ± SD. d Signal intensity of the bands obtained from western blotting of the COL6A1 protein in Ad-MSCs, BM-MSCs, and XF-iMSCs. β-actin was used as the control. Relative band intensity is shown relative to that in XF-iMSCs. Data are shown as the mean ± SD. *p < 0.05; n = 3 (Ad-MSCs, BM-MSCs), n = 2 (XF-iMSCs)