Table 1 Comparison of slow freezing and vitrification methods
From: Effects, methods and limits of the cryopreservation on mesenchymal stem cells
Characteristics | Slow freezing | Vitrification | Â |
|---|---|---|---|
Time | More than 3Â h | Less than 10Â min | |
Speed of freezing | Slow | Fast | |
Sample volume | 100–250µL | 1–2µL | |
Cost | Expensive, freezing machine needed | Inexpensive, no special machine needed | |
Ice crystal formation | Yes | No | |
Post thaw viability | High | High | |
Concentration of CPA | Low | High | |
Risk of CPA toxicity | Low | High | |
Potential pathogen contamination | Low | High | |
Procedure | Simple | Complex | |