Fig. 1

Characterization of bone marrow MSCs. A) Cummulative proliferation of MSCs during 6 passages in vitro. B-C) Tri-lineage differentiation potential and proportion of senescence-associated beta-galactosidase positive cells. Individual MSC responses are summarized in the table (B). Donor responses were evaluated qualitatively as high (+++), positive (++), low (+) and negative (-). Representative images of three-lineage differentiation and positive beta-galactosidase staining are shown for donors F20 and M90 (C). Insets represent cultures in control media. Images for all donors are presented in Fig. S1A. D) Expression levels of surface antigens associated with MSCs. Individual flow cytometry charts for all donors are presented in Fig. S1B