Fig. 2

hiPSC-MP-conditioned medium enhances early osteogenic differentiation of bone marrow MSCs. A-B) DNA content and C-D) ALP activity quantification after 14 days of continuous supplementation with CM or control medium under non-osteogenic and osteogenic conditions. Data is shown for MSCs from all 13 donors relative to week 1 (A, C) and individual donor responses are shown for cultures under osteogenic conditions (B, D). Group labels: CTRL—control, CM—conditioned medium, CTRL + O—control with osteogenic supplements, CM + O—conditioned medium with osteogenic supplements. Data represents individual values with mean (A, C, n = 13) or a combination of mean ± SD with individual values (B, D, n = 3–10). Statistically-significant differences between the groups were evaluated using Friedman test followed by Dunn’s multiple comparisons test (A, C) or unpaired t-test (B, D), and are marked as: *p < 0.05; **p < 0.01