Fig. 3

Osteogenic response of bone marrow MSCs to hiPSC-MP-conditioned medium is dose-dependent. A) DNA content and B) ALP activity quantification after 14 days of continuous supplementation of MSCs (donors F71, F85, M89) with increasing doses of CM or control medium under non-osteogenic and osteogenic conditions. Group labels: CTRL—control, CM/8, CM/4, CM/2—conditioned medium dilutions 1:8, 1:4, 1:2, CM—conditioned medium, CTRL + O—control with osteogenic supplements, CM/8 + O, CM/4 + O, CM/2 + O—conditioned medium dilutions 1:8, 1:4, 1:2 with osteogenic supplements, CM + O—conditioned medium with osteogenic supplements. Data represents a combination of mean ± SD with individual values (n = 4–5). Statistically-significant differences between the groups were evaluated using one-way ANOVA followed by Tukey’s multiple comparisons test and are marked as: *<0,05; **<0,01; *** <0,001; ****<0,0001