Fig. 4

hiPSC-MP-conditioned medium outperforms bone marrow MSC-conditioned media. A) DNA content and B) ALP activity quantification after 14 days of continuous supplementation of MSCs (donors F71, F85) with hiPSC-MP-CM, CM from MSCs F20, F71 and F85 or control medium under non-osteogenic and osteogenic conditions. Group labels: CTRL—control, hiPSC-MP—conditioned medium from hiPSC-MPs, MSC M22/F71/F85—conditioned medium from M22/F71/F85 cells, CTRL + O—control with osteogenic supplements, hiPSC-MP + O—conditioned medium from hiPSC-MPs with osteogenic supplements, MSC M22 + O/F71 + O/F85 + O—conditioned medium from M22/F71/F85 cells with osteogenic supplements. Data represents a combination of mean ± SD with individual values (n = 3–4). Statistically-significant differences between the groups were evaluated using one-way ANOVA followed by Tukey’s multiple comparisons test and are marked as: *<0,05; **<0,01; *** <0,001; ****<0,0001