Fig. 8

Factors secreted by cells in vitro and striatal expression of BDNF and FKN in vivo. a While GDNF levels only tended to rise, concentrations (pg/ml) of BDNF, FKN, SCF, VEGF-A, and VEGF-C, were all increased in differentiation compared to proliferation CM (GDNF P = 0.0750; BDNF ***P = 0.0002; FKN ****P < 0.0001; SCF **P = 0.0092; VEGF-A *P = 0.0355; VEGF-C ***P = 0.0002). Two-tailed unpaired t-tests. 20/20 + n = 2, Lotharius n = 2, Proliferation CM n = 3, Differentiation CM n = 8 except for GDNF where n = 7. ns = not significant. b Four months post-transplant, in the rostral Str, hNSC-transplanted mice tended to have increased BDNF immunostaining compared to those which received buffer. Control n = 3, MPTP + buffer n = 3, MPTP + cell n = 4. Scale bar = 200 μm. c Striatal FKN expression was similar across all experimental groups four months post-transplant. Control n = 3, MPTP + buffer n = 4, MPTP + cell n = 4. b, c One-way ANOVA followed by Tukey´s post-hoc test. Scale bars = 200 μm. CM = conditioned media, hNSC = human neural stem cell, Str = striatum