Table 2 Cell lines and culture parameters to produce cell-conditioned medium

Human

Adipose tissue

Mesenchymal stem cells

Primary cells, home-made

Hypoxia (37 °C, 1% oxygen and 5% CO₂ for 48 h)

P4 - P6

[60]

Murine (male C57BL/6 or mRFP1 mice)

Bone marrow

Monocytes

Primary cells, home-made

Monocyte polarization to M0 (no additives), and M1, M2a, M2c macrophage due to cytokine (LPS, IFN-g, IL-4 13, IL-13, IL-10) addition during 24 h

-

[53]

Human

Bone marrow

Mesenchymal stem cells

Self-renewing cells; PT-2501, Lonza

-

-

[54]

Human

Umbilical cord

Mesenchymal stem cells

Transformed cells, primary cells

The cells were transfected to produce hCAP-18/LL-37

P4-P6

[55]

Human

Redundant skin tissue samples from abdominoplasty or face-lift surgery

Dermal fibroblasts

Primary cells

-

P3

[61]

Human

Umbilical cord

Mesenchymal stem cells

Primary cells

-

Several P cycles

[64]

Human

Placentas from parturients after cesarean section

Clusters of cells comprising amnion

Primary cells

-

P0

[63]

Human

Adipose tissues

Mesenchymal stem cells

Primary cells

-

P3

[56]

Human

Placentas from parturient after cesarean section

Mesenchymal stem cells

Primary cells

Hypoxia (1% oxygen)

P1

[72]

Murine

Tumor in a male mouse induced with the Abelson murine leukemia virus

Monocyte/

macrophage

Transformed cells, RAW 264.7 from the cell bank of Chinese Academy of Sciences Typical Culture Preservation Committee

Bioglass ion extracts (1/128) added to the culture medium to polarize monocytes to M2-macrophages

-

[57]

Human

Adipose tissue

Mesenchymal stem cells

Primary cells

-

P 6–8

[52]

Human

Kidney of a human embryo

Epithelial cells

Transformed cells; HEK293, the National Cell Bank of Iran

The cells were transfected with pcDNA3.1-NRF2 plasmid

P5, P15, P20

[65]

Sheep

Skin biopsy

Dermal fibroblast; Ovine epidermal keratinocytes

Primary cells

Fibroblasts and keratinocytes were initially co-cultured to obtain highly pure both cell types

P3

[62]

Human

Adipose tissue

Stromal cells

Primary cells

-

P3-P6

[71]

Human

Placenta tissue

Multipotent mesenchymal stromal cells

Primary cells

-

P3

[59]

Human

Umbilical cords

Mesenchymal stem cells

Primary cells

-

P3-P6

[69]

Human

Blood

Mononuclear cells

Primary cells

Cells were treated by γ-irradiation (2 × 30 Gy) to induce apoptosis/necroptosis

-

[78, 94]

Rat

Nasal mucosa

Ecto-mesenchymal stem cells

Primary cells

-

-

[66]

Human

Adipose tissue

Mesenchymal stem cells

Transformed cells; hTERT immortalized SCRC-4000), ATCC

-

P6-P10

[70]

Human

Skin

Multipotent stromal cells

Primary cells

The cells were tested to check viability and phenotypic changes before and after cultures, remaining viable and phenotypically unchanged during the assays

-

[67]

Human

Peripheral blood from an acute monocytic leukemia patient

Monocyte/

macrophage

Transformed cells, THP-1, ATCC

Monocyte polarization to M0, M1, M2-macrophages adding PMA, LPS, IFN-g, IL-4 and IL-13

-

[58]