Fig. 2

Representative histopathological images of the brain coronal sections and the heart four-chamber sections. A–C HE staining and Nissl staining results of the frontal lobe in different groups. D Statistical analysis of Nissl staining in the frontal lobe (The statistical method involves converting positive staining into grayscale, calculating the grayscale value and area, and determining the Nissl staining quantification result for each sample by dividing the grayscale value by the area, using Python 3.7). E–G HE staining and Nissl staining results of the hippocampus in different groups. H Statistical analysis of Nissl staining in the hippocampus (The statistical method involves converting positive staining into grayscale, calculating the grayscale value and area, and determining the Nissl staining quantification result for each sample by dividing the grayscale value by the area, using Python 3.7). I–K Cross-sectional HE-stained images of heart tissue. HE staining of heart tissue with red arrows in J-2 indicating disorganized sarcomeres in the myocardium of SAMP8 mice. L Statistical analysis of the minimum ventricular wall thickness measured on panoramic scanned images of HE-stained sections using Qupath software. n = 7 ~ 8 per group. The pathology images in the figure were representative images of animals euthanized on the 78th day after the first HUCMSCs injection. Data were analyzed using one-way ANOVA followed by Tukey's post-hoc test in subfigure D, H, L. Abbreviation: R1_P: SAMR1_PBS; P8_P: SAMP8_PBS; and P8_M: SAMP8_MSC