Fig. 3

CD141 is a critical marker that distinguishes BM-VPCs from BM-MSCs. A List of markers with an expression difference of > 50% between BM-VPCs and BM-MSCs screened among 376 surface markers. B Differential expression of CD141 and CD282 in 15–20 donors assessed using flow cytometry analysis (***p < 0.001, Student's t-test, two-tailed). C–F BM-MNCs were sorted using magnetic bead-conjugated CD141 antibody. The selected MNCs were plated in EGMPL. C Colonies that appeared from CD141⁺ sorted BM-MNCs on days 5, 7, and 9. Scale bar = 500 μm. D Marker expression profile of ex vivo-cultured cells from CD141⁺ sorted colonies at passages 1 to 4 analyzed using flow cytometry. E, F CD141⁺ sorted BM-MNCs were cultured in EGMPL at passage 0; from passage 1 to 3  and then, the cells were divided and cultured in EGMPL or StemMACS. E Morphology of replated CD141⁺ sorted cells in EGMPL and StemMACS at passages 1 to 3, and their tube-forming ability in MEMα + 0.2%hPL at passage 2. Scale bar = 500 μm. F Marker expression profile of CD141⁺ sorted cells cultured in StemMACS