Fig. 3

CXCL1 is a key factor involved in the aggravation of liver injury in APAP-induced ALF. A) Schematic illustration of the experimental design. B) Representative images of HE-stained liver tissue from the indicated groups of mice. Scale bar = 100 μm. C) The percentage of the liver necrosis area (n = 5/group). D) Statistical graph of the serum AST and ALT levels in each group of mice (n = 6/group). E-F) Representative dot plots of neutrophils (CD11b⁺Ly6G⁺) in mouse liver tissue detected by flow cytometry and the corresponding statistical data. (n = 5/group). G) Bar graph of neutrophils (CD11b⁺Ly6G⁺) in the mouse spleen detected by flow cytometry (n = 3/group). H) The mRNA expression levels of Cxcl1 in the liver tissue of the indicated groups of mice were detected by RT‒qPCR (n = 6/group). I) The mRNA expression levels of Il6 in the liver tissue of the indicated groups of mice were detected by RT‒qPCR (n = 4/group). J) The mRNA expression levels of Tnfa in the liver tissue of the indicated groups of mice were detected by RT‒qPCR (n = 6/group). K) Representative images of Ly6G immunohistochemical staining of liver tissue from the indicated groups of mice. Scale bar = 100 μm. L) Representative images of Cxcl1 immunohistochemical staining of liver tissue from the indicated groups of mice. Scale bar = 100 μm