Fig. 4

MSC-EVs alleviate liver injury and neutrophil infiltration in vivo. A) Schematic illustration of the experimental design. B) Representative images of HE-stained liver tissue from the indicated groups of mice. Scale bar = 100 μm. C) The percentage of the liver necrosis area (n = 5/group). D) Statistical graph of the serum AST and ALT levels in the indicated groups (n = 6/group). E-F) Representative dot plots of neutrophils (CD11b⁺Ly6G⁺) in mouse liver tissue detected by flow cytometry and the corresponding statistical data (n = 5/group). G) Bar graph of neutrophils (CD11b⁺Ly6G⁺) in the mouse spleen detected by flow cytometry (n = 3/group). H) The mRNA expression levels of Cxcl1 in the liver tissue of the indicated groups were detected by RT‒qPCR (n = 3/group). I) The mRNA expression levels of Il6 in the liver tissue of the indicated groups of mice were detected by RT‒qPCR (n = 6/group). J) The mRNA expression levels of Tnfa in the liver tissue of the indicated groups of mice were detected by RT‒qPCR (n = 6/group). K) Representative images of Ly6G immunohistochemical staining of liver tissue from the indicated groups (scale bar = 100 μm). L) Representative images of Cxcl1 immunohistochemical staining of liver tissue from the indicated groups of mice (scale bar = 100 μm)