Fig. 6

miR-186-5p plays a key role in MSC-EV therapy for APAP-induced liver injury. A) Venn diagram showing the intersection between potential miRNAs predicted by bioinformatics websites to bind with Cxcl1 and the existing sequencing data. B) The potential binding site sequence of miR-186-5p and CXCL1. C) Bar graph representing the fluorescence intensity of HEK293T cells transfected with luciferase reporter plasmids determined via the Dual-Luciferase Reporter Assay System (n = 4/group). D) Representative dot plots of Annexin V and PI staining of the indicated groups detected by flow cytometry. E) Bar graph showing the statistical data of the number of Annexin V⁺ cells (left, n = 9/group) and the number of Annexin V⁻PI⁻ cells (right, n = 9/group). F) Schematic diagram of the neutrophil Transwell migration test of miR-186-5p mimic transplantation for the treatment of the APAP-induced AML12 cell injury model. G) Bar graph displaying the chemotactic number of CFSE-labeled neutrophils (n = 3/group)