Fig. 4

Immunofluorescent detection of HLA-I and HLA-II expression in gene knockdown DPSCs. The cells were fixed using 4% PFA. a Immunofluorescence images (× 20) of HLA-I and HLA-II expression in DPSCs with genetic modification. The HLA-I and HLA-II were stained with red and green fluorescence, respectively. The scale bar represents 200 μm. b, c Quantification of HLA-I and HLA-II mean fluorescence intensity in genetically modified DPSCs with or without the IFN-γ treatment. d, e Quantification of HLA-I and HLA-II positive cell numbers in genetically modified DPSCs with or without the IFN-γ treatment. ns = no significant difference. *p < 0.05, **p < 0.01, ***p < 0.001