Fig. 6

mRNA profiling data demonstrates that freshly isolated dermal papilla cell (fDPCs) and traditionally isolated dermal papilla cell (tDPCs) are transcriptionally dissimilar. A Heatmap showing relative expression levels of 56 progressively downregulated differentially expressed genes (DEGs) and 30 progressively upregulated DEGs. Relative expression levels are colour-coded as colour key. B Principal component analysis of gene expression profiles of nine samples (three each of fDPC-P0, DPC-P0, and DPC-P3). C Pearson rank correlations were computed for every pair of transcriptome profiling samples. Resulting colour-coded correlation matrix revealing similarity of transcriptomes of fDPCs and tDPCs. D, E Expression patterns representing genes that were progressively up- or downregulated. F Venn diagram showing 148 overlapping DEGs by at least twofold in fDPC-P0, tDPC-P0, and tDPC-P3. G KEGG pathway enrichment for 148 DEGs. H RT-qPCR validation of mRNA expression of fDPCs and tDPCs. Abundances of mRNAs were normalized to that of GAPDH