Fig. 3

Single-cell Transcriptomic Analysis Reveals Four Major Subpopulations within MSCs. (A) tSNE plot illustrating the clustering of 10,463 cells from MSC-GY (above), along with the statistical analysis indicating the distribution of cells in each subpopulation (below). (B) Heatmap displaying the top 10 marker genes for each subpopulation. (C) GSEA-KEGG showing the enriched pathway for each subpopulation. (D) tSNE plot demonstrating the distribution of each subpopulation across the cell cycle. (E) Pseudotime analysis reflecting the cell state transition and differentiation trajectories of these four subpopulations