Fig. 1

Unopposed mitochondrial fusion, cellular senescence and fibrosis occur successively in the uIRI murine model. A Schematic illustrating the collection of animal specimens at different indicated time points. Unilateral IRI was performed in C57BL/6 mice and euthanized separately on days 0, 2, 7, 14, and 28 (n = 6). B Images of Masson staining. Scale bar: 50 μm. C Images illustrated the overall distribution of the SA-β-gal-positive cells. Scale bar, 50 μm. D Immunofluorescence staining images of the mitochondrial outer membrane (Tomm20). Scale bar, 20 μm. E Western blot bands of senescence (upper), fibrosis (middle), and mitochondrial dynamics markers (bottom). F Quantitative immunoblot analyses of the above bands. The quantification of phosphor-drp1 (Ser616 or Ser637) was normalized to total-drp1. Data are presented as mean ± SD. **P < 0.01; *P < 0.05. ns, not significant