Fig. 2

Effect of POU3F4 on proliferation of hippocampal NSCs. A Immunostaining for Ki67 (red) in WT mouse hippocampal NSCs which were infected with LV-POU3F4 or LV-Ctrl. Nuclei were stained with Hoechst (blue). B Quantification of Ki67/GFP-positive cells in the LV-Ctrl and LV-POU3F4 groups in A. C Morphology of neurospheres of hippocampal NSCs obtained from the WT and POU3F4^−/− mice at different days of culture. D–F Quantification of the number of neurospheres with different diameters in the 3rd d (D), 7th d (E) and 14th d (F) of culture. n = 3. Scale bar = 100 µm in (A). Scale bar = 400 µm in (C). * P < 0.05, *** P < 0.001 vs LV-Ctrl or WT