Fig. 3

Effects of POU3F4 on the DG neurogenesis and behavior of learning and memory. A Immunofluorescence staining against DCX in the DG of WT and POU3F4^−/− mice (3 and 5-week-old). The arrows showed DCX positive cells. The boxes in the corners of the figures showed locally enlarged images. B Statistical analysis of the number of DCX positive cells in each group. n = 3. Scale bar = 400 µm. experiment respectively. The tested indexes were as follows: the latency time that mice took to find the target platform, the latency time that mice first arrived at the target area, and the number that mice passed through the target area. C, D, E The WT and POU3F4^−/− mice at 1 (C), 2 (D) and 5 (E)-month old age were subjected to Morris water maze. Left, middle and right panels are respectively the statistical analysis of the time to find platform, latency time to cross the target area and the number of times mice entered the target quadrant. F, G The WT and POU3F4^−/− mice at 5-month old age were subjected to passive (F) and active (G) avoidance task. C, n = 13; D, F, G, n = 8; E, n = 5. *P < 0.05, **P < 0.01, ***P < 0.001 vs WT