Fig. 6
Gli1 promoted hippocampal NSCs to differentiate into neurons. Single hippocampal NSCs were seeded in a 48-well plate at a density of 1 × 104 cells/well and followed with infection of LV-Ctrl, LV-Gli1, shCtrl or shGli1 for 12 h and subsequent culture in differentiation medium for 7d. A Immunocytochemical staining against GFAP, Tuj1, and MAP2 antibodies (all are red) after induced differentiation of hippocampus NSCs transfected lentivirus-Gli1. B–D Quantification of the GFAP (B), Tuj1 (C) and MAP2 (D) positive cells in A. (E) Immunocytochemical staining against GFAP, Tuj1 and MAP2 antibodies (all are red) after induced differentiation of hippocampus NSCs transfected shGli1. F, G and H Quantification of the GFAP (F), Tuj1 (G) and MAP2 (H) positive cells in E. Nuclei were stained with Hoechst (blue). n = 3. ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs control. Scale bar = 200 µm