Fig. 7

Gli1 reversed proliferation increase and neuronal differentiation inhibition of the POU3F4-knockout hippocampal NSCs. Hippocampal NSCs derived from WT and POU3F4^−/− mice were infected with lentiviruses and induced to differentiate, and then subjected to immunocytochemical analysis. A, B, C Immunostaining showing the MAP2 (A), Tuj1 (B), Ki67 (C)-positive cells (red). D, E, F Quantification of the proportion of the above corresponding positive cells in A, B and C. Nuclei were stained with Hoechst (blue). n = 3. * P < 0.05, *** P < 0.001. Scale bar = 100 µm