Fig. 2

Extension and evaluation of SCAPs. A Image of primary SCAPs cells extracted and cultured to P3, observed under a 4 × microscope, showing the typical spindle shaped MSCs morphology for all types of cells. Scale bars = 100 μm. B Flow cytometry was used to determine the expressions of hematopoietic stem cell markers CD34, CD45, and HLA-DR on the surface of SCAPs, as well as the expressions of mesenchymal markers CD73, CD90 and CD105. C Images showing in vitro trilineage differentiation of cultured SCAPs. The trilineage differentiation capacities of the SCAPs were confirmed by alizarin red for extracellular calcium. Oil red O for intracellular lipid accumulation, and Alcian blue for extracellular cartilage ( Scale bars = the left and the right is 100 μm; the middle is 50 μm)