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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: AGEs impair osteogenesis in orthodontic force-induced periodontal ligament stem cells through the KDM6B/Wnt self-reinforcing loop

Fig. 2

KDM6B broadly regulates the downstream transcriptome induced by mechanical stimulation. (a) Volcano plot of DEGs following OE-KDM6B treatment. (b) Heatmap of the top 20 upregulated and downregulated DEGs. (c) Expression differences in periodontal collagen-related genes. (d) Venn diagram showing common upregulated DEGs between the OE-KDM6B and GSE112122 datasets. (e) GO analysis of common DEGs, illustrating BP, CC, and MF terms. (f) KEGG pathway analysis of common DEGs. (g) ChIP-Seq analysis showing H3K27Me3 enrichment at transcription start sites and within gene bodies. (h) PPI network identifying an osteogenesis-related subnetwork. (i) IHC showing the effects of orthodontic force and GSK-J4 on the expression of osteogenesis-related markers (Alp and Runx2, n = 5). Data are presented as mean ± SD. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512