Fig. 2

Establishing an acute inflammation model with low-dose LPS. (A) C57BL/6 mice were SC-injected with 30 ng or 100 ng LPS in 50 µl PBS in the left hock. At 0, 4, 8, 24 h post-LPS injection, skin tissue around the LPS injection site was excised and homogenised for cytokine analysis. Changes in pro-inflammatory TNF, IL-1β and MCP-1 in the skin tissues over the time course. n = 3 mice per timepoint from one experiment. (B) Draining LNs were harvested from mice at 0, 4, 8, 24 h after SC injection of 30 ng LPS in 50 µl PBS in the left hock. Total LN, neutrophil and monocyte cellularity at various timepoints post-LPS injection. (C) Inflammatory gene expression in whole LN cell lysate and sorted LN neutrophils and monocytes at 24 h post-LPS injection. Fold-change differences in mRNA expression of inflammatory genes were plotted after normalising mRNA expression level to housekeeping genes (GAPDH and β-actin). Data expressed as mean ± SEM; UNT (untreated LN) n = 2–3 mice per timepoint; LPS (LPS dLN) n = 3–4 mice per timepoint