Fig. 1
Using MRR system to measure changes in T2 when iPSCs differentiate into SCPCs. A MRR system consists of a 0.5 T permanent and portable magnet that has a radiofrequency (RF) detection probe hooked up to an RF spectrometer. iPSC and SCPC samples of 4 µL consisting of about 180,000 cells are loaded into a microcapillary tube. The microcapillary tube is sealed with critoseal and fitted into the RF probe with the sample directly within the RF detection coil. The actual RF probe is shown on the right of the schematic for illustration purposes. B Illustration of CPMG (Carr-Purcell-Meiboom-Gill) pulse sequence for obtaining T2 measurements. C Illustration of spin–spin relaxation time of iPSCs changing as they differentiate into SCPCs. iPSC and SCPC cell figures are adapted from the icon library of “Cell Types” by BioRender.com