Fig. 4
T2 decreased with increasing OCT4 levels in day 10 SCPCs corresponding with manufacturing considerations, including batch-to-batch, operator and cell-line variations. A Correlation of day 10 SCPC T2 values with the percentage of day 10 OCT4+ cells quantified by immunofluorescent staining. 3 different operators generated SCPCs, and each data point refers to a different batch of SCPCs that was generated. At least 10 ROIs (more than 100,000 cells) were quantified for each experimental repeat (n = 12). B Day 10 BJ-SCPCs had lower OCT4 levels and higher T2 values as compared to day 10 CLEC23 SCPCs. At least 25 ROIs (more than 60,000 cells) were quantified for each experimental repeat (n = 9)