Fig. 7
Day 10 SCPCs with T2 higher than 1700 ms has better extent of differentiation to Day 28 spinal cord motor neurons. A Day 10 SCPCs were differentiated into Day 28 spinal cord motor neurons and compared both T2 and Day 28 motor neuron marker expression. Cell figures were adapted from the icon library of “Cell Types” by BioRender.com. B Immunofluorescent staining of ISL-1 and SMI-32 markers for identification and quantification of motor neurons. A motor neuron is stained positive for both ISL-1 and SMI-32 (Scale bar: 50 µm). C Increasing level of expression in Day 28 motor neurons found in batches of day 10 SCPCs that had T2 values higher than 1700 ms as quantified by immunofluorescent staining. Red line separates the high and low motor neuron expressing groups. At least 25 ROIs (more than 80,000 cells) were quantified for each experimental repeat (n = 7)