Fig. 1

FLT3LG and SCF caused floating cells to appear from hHFMSCs^OCT4. (a) Schema showing the transformation of floating cells derived from hHFMSCs^OCT4 into erythroblasts. (b) Cell morphology changes and floating cells observed under immunofluorescence microscope, with a floating cell indicated by a yellow arrow and a floating cell colony outlined by a dashed circle. (c) Phase contrast microscope used to observe floating cells’ morphology. Floating cell indicated by yellow arrow. (d) Collecting and re-cultivating suspended cells. (e and f). Floating cell colonies were selected to identify CD45 expression, with green indicating EGFR, red indicating PE-CD45, and blue indicating Hoechst. Peripheral blood cells served as a positive control