Fig. 4

The difference in self-renewal and proliferative ability between the adherent hHFMSCs^OCT4 and floating hHFMSCs^OCT4. (a) Cell proliferation curve of hHFMSCs, adherent hHFMSCs^OCT4 and floating hHFMSCs^OCT4 obtained from the CCK-8 assay (n = 3). (b) Colony formation assay of hHFMSCs, adherent hHFMSCs^OCT4, and floating hHFMSCs^OCT4; the enlarged views show the difference between the three cell clones (n = 3). The histogram is the rate of colony formation for each cell. (c) ICC of proliferation-associated protein Ki67 expression in three cell groups, and Ki67 protein was localized in the nucleus (n = 3). *P˂0.05, **P˂0.01, ****P˂0.0001. (d) Discrete cutoffs for distinct cell cycle phases are visually selected to calculate the percentages of cells in G1, S, and G2/M phases using R software