Fig. 6
Expression of AJ core genes and target genes in floating hHFMSCsOCT4. (a) RT‒qPCR analysis of the expression of ZO-1, ACTN2, E-cadherin, β-catenin, and Cyclin D1 (n = 3). (b) IF staining images showing the location of the β-catenin protein in hHFMSCs, adherent hHFMSCsOCT4, and floating hHFMSCsOCT4. (c) Fluorescence intensity of the merged colour in the nucleus was measured in three groups using ImageJ. *P˂0.05, **P˂0.01, ***P˂0.001