Table 2 Optimal dilutions of antibodies used for Western blot/IF analysis
Antibodies | Dilution rates |
|---|---|
Mouse Anti-Human β-Actin antibody (Bioss) | 1:5000 |
Mouse anti-human PE-CD45 (BD Pharmingen) | 1:100 |
Rabbit Anti-Mouse ZO-1 antibody (Cell Signaling Technology) | 1:2500 |
Mouse Anti-Human OCT3/4 Ready-to-Use antibody (Maxim) | 1:4 |
Mouse Anti-Human β-catenin antibody (Maxim) | 1:1500 for Western blot,1:150 for IF |
Goat Anti-Rabbit IgG/HRP (Bioss) | 1:3000 |
Goat Anti-Mouse IgG (H + L) (Elabscience) | 1:10000 |
Goat anti-mouse Alexa Fluor 594-conjugated IgG (Abcam) | 1:200 |