Fig. 3

The expression levels of MET and stemness-related genes and proteins were decreased in Epi-GMSCs. (A) RT-qPCR analysis of the expression of N-cadherin, Vimentin, KLF4, SOX2, OCT4, and NANOG after days 3, 9, and 15 of incubation in GECCM (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (B) WB and (C) quantitative analysis of the expression of N-cadherin, Vimentin, KLF4, and SOX2 after days 3, 9, and 15 of incubation in GECCM. Full-length blots/gels are presented in Supplementary Fig. 2. (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001). (D) Quantitative analysis of KLF4 immunofluorescence intensity using ImageJ. (n = 3, **P < 0.01). (E) Representative confocal fluorescence images showing the subcellular localization of KLF4 (red). Cytoskeleton analysis was performed by immunofluorescence staining with F-actin (green). (n = 3)