Fig. 4

The expression levels of epithelial-related genes and proteins were elevated in Epi-GMSCs. (A) RT-qPCR analysis of the expression of KRT12, KRT15, KRT19, KRTAP2-3, and ITGB3 after days 3, 9, and 15 of incubation in GECCM (n = 3, *P < 0.05, **P < 0.01, ****P < 0.0001). (B) WB and (C) quantitative analysis of the expression of KRT12, KRT15, KRT19, and E-cadherin after days 3, 9, and 15 of incubation in GECCM. Full-length blots/gels are presented in Supplementary Fig. 3. (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001). (D) Representative confocal fluorescence images showing the subcellular localization of KRT19 (red, white arrows). Cytoskeleton analysis was performed by immunofluorescence staining with F-actin (green). GECs served as a positive control for high KRT19 protein expression. (n = 3)