Fig. 7

Inhibition of the Wnt signaling pathway promoted the expression of epithelial-related genes. (A) Representative images of GMSCs captured under an inverted phase contrast microscope after 3 days of incubation in GECCM with and without MSAB (5 µM) (n = 3). (B) RT-qPCR and (C, D) WB analysis of the expression of β-catenin after 3 days of incubation in GECCM with and without MSAB (5 µM) (n = 3, ^nsP > 0.05, *P < 0.05). Full-length blots/gels are presented in Supplementary Fig. 8. (E) RT-qPCR analysis of the expression of KRT12, KRT15, KRT19, LRP5, RUNX2, and COL1A1 after 3 days of incubation in GECCM with and without MSAB (5 µM) (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)