Fig. 3
Differential cellular uptake of fluorescently labelled and mRNA-loaded mimetics. a Fluorescence microscope images showing DiD-labelled mimetics taken up by two cell lines, murine macrophages (J774) and human fibroblasts (MRC-5) at early time points (4, 8 and 12 h). (Magnification: 10X, Scale bar:100 µm). b Graph showing mRNA-GFP encapsulation efficiency (EE) in MIMs and EXOs as revealed by Quant-it™ RiboGreen RNA Assay Kit. (n = 3). Time-dependent appearance of GFP fluorescence expressed as percentage of GFP-positive cells in c macrophages and d fibroblasts observed by flow cytometry after 24 h, 48 h and 72 h post transfection with MIMs. EXOs are used for comparison. (Data represented as mean ± SD, n = 4). Quantification of changes in the mean fluorescent intensity detected in macrophages e and fibroblasts f at each time point. Untreated cells used as baseline. (n = 4, p*** < 0.001)