Fig. 4
Mimetics and exosome biodistribution in whole embryo culture. a Schematic representation of whole embryo culture established to define mimetics (MIMs) and exosomes (EXOs) distribution ex vivo. Embryos were isolated from pregnant dams and cultured in presence of MIMs or EXOs (1 × 108) administered in culture media for 24 h. Confocal microscopy images showing explanted yolk sac, b and embryos, c upon exposure to DiD-labelled (red), mRNA-GFP-loaded (green) MIMs or EXOs. Phalloidin (gray) and Hoechst (blue) were used to counterstain actin filaments and DNA, respectively. Scale bars: 500 µm