Fig. 4

Results of immunofluorescence double staining of rectus femoris muscle in rats. (A, B) Representative photographs of immunofluorescence double-stained sections of rectus femoris muscle in each group (DAPI-labeled nuclei were in blue color, Desmin-labeled muscle satellite cells are in green color, and Ki-67-labeled cells were in red color for cell proliferation, and Desmin and Ki-67 were co-expressed as the number of proliferating muscle satellite cells, scale bar = 50 μm). (C, D) The number of muscle satellite cell proliferation in immunofluorescence double-stained sections of rectus femoris muscle of rats in each group after 10 and 20 days of traction treatment (n = 6). As the staining was performed on harvested tissue, it is possible that more cellular proliferation would have been present in vivo. ^*P < 0.05 versus group N. ^#P < 0.05 versus group M. ⁺P < 0.05 versus group R