Fig. 5

Results of HE staining and Masson staining of rat rectus femoris muscle. (A-D) Representative photographs of HE stained and Masson stained sections of rectus femoris muscle of rats in each group (scale bar = 200 μm). Nuclei were blue and cytoplasm was red in HE staining; collagen fibers were blue in Masson staining; myofibrils, fibrillar pigment and erythrocytes were red. (E, F) The results of CSA of myofibers in HE stained sections and the ratio of collagen fiber area to muscle fiber area in Masson stained sections were counted after 10 and 20 days (areas of collagen fiber deposition were indicated in blue, n = 6). *P < 0.05 versus group N. #P < 0.05 versus group M. +P < 0.05 versus group R