Fig. 5

Magnesium ions target PFKFB3 to regulate glycolytic metabolism, promoting ENCCs differentiation into neurons. A–C WB and PCR confirm the efficiency of ENCCs overexpression of PFKFB3 (n = 3) (Full-length blots/gels are presented in Supplementary Material 16E). D Overexpression of PFKFB3 in ENCCs leads to increased expression of HK2, LDHA, and GLUT (n = 3). E, F Overexpression of PFKFB3 in ENCCs using neurogenic induction media for 7 days results in a significant upregulation of TUJ1 and UCHL1 (n = 3). G Surface plasmon resonance results show a strong binding relationship between magnesium ions and biotin-labeled PFKFB3, with a kDa of 1.08 μM. NC: negative control. Data were presented as mean ± SEM. *p < 0.05; **p < 0.01