Fig. 2

Human Nestin ((h)Nestin) stem cell marker expression decrease after 21 days of neurodifferentiation. (A) hDPSCs proliferated in serum containing media, showing fibroblast like morphologies and a detectable decrease of (h)Nestin labelling (green) after 21 days of differentiation. Scale bar 50 µm. (A’) Quantification of (h)Nestin (green) positive cells in cultures obtained from 3 different donors just after switching them from a DMEM-10% FBS media to a neural induction mix and 21 days afterwards. ***p < 0.001 (B) Cells grown in neurocult proliferation mix presenting a ramified morphology and a decrease in (h)Nestin positive labelled cells (green) after 21 days of differentiation. All images are counterstained with DAPI. Scale bar 50 μm. (B’) Percentage of (h)Nestin positive cells in cultures obtained from 3 different donors after switching them from a serum-free proliferation mix into a neural induction mix and 21 days afterwards. ***p < 0.001. Mann-Whitney U test (Two-tailed)