Fig. 3

Glial fibrillary acidic protein (GFAP) expression decrease in hDPSCs after 21 days of neural induction. (A) hDPSCs grown with serum stained with GFAP antibody (red) at the beginning of the neural differentiation process and 21 days thereafter. Scale bar 50 µm. (A’) Graph showing the decrease of GFAP positive cells in hDPSCs expanded with DMEM-10% FBS after switching them to a neural induction mix. ***p < 0.001 (B) Fluorescence photomicrographs of GFAP (red) in hDPSCs expanded in serum-free Neurocult proliferation mix just after changing them to a neural induction mix and 21 days after. All images are counterstained with DAPI. Scale bar 50 μm. (B’) Graph showing GFAP positive cell percentage reduction 21 days after switching cultures from Neurocult proliferation mix to neural differentiation media. Cell cultures were obtained from 3 different donors. Data shown as mean ± SD. ***p < 0.001. Mann-Whitney U test (Two-tailed)