Fig. 4

Neurodifferentiated hDPSCs are able to commit toward Schwann Cell phenotypes. Venn’s diagrams representing the percentage of positive cells for S100β and p75^NTR glial markers after 21 days differentiation, as well as the percentage of co-localizing cells for hDPSCs grown with FBS (A) and those that were not (B). (C) Graphs showing the percentage difference for S100β and p75^NTR markers between hDPSCs that have grown with FBS and those in serum-free Neurocult media after switching them to the same neural induction mix during 21 days. hDPSCs cultures obtained from 3 different donors.**p < 0.01 (D) Higher rates of immunopositive cells for p75^NTR (red) and S100β (green) could be observed in hDPSCs grown with Neurocult comparing to those that were grown in FBS after 21 days of differentiation. Scale bar 50 μm. Data shown as mean ± SD. Statistical analyses were conducted by Mann-Whitney U test (Two-tailed)