Fig. 5

Neuronal marker expression and cell morphological analysis after 21 days of neurodifferentiation. (A) Immunofluorescence images showing doublecortin (DCX) and neuronal nuclear protein (NeuN) positive labelling in hDPSCs and different morphologies observed between cells grown in a serum containing medium and those that were grown in a serum-free medium. Scale bar 50 μm. 3D Sholl-analysis of 30 cells from cultures from 3 different donors per condition revealed longer branch lengths measured in µm (B) and a larger overall surface (µm2) (C) covered by hDPSCs processes in those cells previously grown as a floating dentospheres. Data shown as mean ± SD. ***p < 0.001. Statistical analysis conducted by Mann-Whitney U test (Two-tailed)